Multiple bands on DGGE

j.r.stephen mbi071 at abdn.ac.uk
Mon Jan 8 09:02:13 EST 1996


Hi All,

I've been trying to use DGGE for various purposes recently, but on occation,
even when starting with a clone template, I see multiple bands appearing close
together on the finished gel. Does anyone have experience of this, or any idea
of a solution? One PCR primer carries an extensive GC-clamp,

Thanks, all,

John



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