T/A cloning

Helen Korsmo Helkor at facstaff.wisc.edu
Mon Jan 8 08:15:19 EST 1996


We ligated an insert into a T-vector (after treating the insert for 30 minutes with Taq; in order to 
put A's on the ends) and transformed DH5-alpha cells and plated out on LB plate with IPTG and 
X-gal.  We got several white colonies ( presumably these have insert in the vector), but mostly 
blue (vector alone).  Is it possible for T-vectors to ligate?




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