PCR and DMSO

john brennand john.brennand at gbapr.pharmaceuticals.zeneca.tmailuk.sprint.com
Tue Jan 9 09:28:16 EST 1996


Yoshi

I'm not sure but I believe it helps in the denaturing step - 
particularly good for GC rich regions.  We can usually only get such 
regions to PCR with DMSO - it does however lead to a lowering of 
fidelity and more "errors" in the cloned PCR products 

John





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