PCR and DMSO

Patrick HJ Falckh p.falckh at rmit.edu.au
Tue Jan 9 19:45:41 EST 1996


DMSO is suppose to reduce secondary structure of the target DNA, which 
would be benificial, however, it also inhibits Taq polymerase and 
therefore the yield will be lower: Randall Saiki wrote this in his chapt 
on "The Design and Optimisation of the PCR" from "PCR Technology: 
Principles and Applications for DNA Amplification" ed HA Erlich, Freeman  
& Co, NY, 1991.

Regards

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