PCR on whole blood

[Patrick HJ Falckh]

horton at biosci.cbs.umn.edu (Robert Horton) wrote:
>>Lo Wing Ip Anthony (alo at b2.hkstar.com) wrote:
>>: Hi,
>>
>>: 	As a diagnostic lab, I would like to perform PCR type of 
>>: amplification on whole blood in order to avoid extra step for the 
>>: technicans. I come across an article of using Tth for that. Just wonder 
>>: whether anyone had an experience with this polymerase?
>>
>This isn't about Tth, but if you're interested in PCR from whole blood, you
>should see
>
>    Walsh PS.  Metzger DA.  Higuchi R.                                         
>    Chelex 100 as a medium for simple extraction of DNA for PCR-based typing   
>    from forensic material.                                                    
>    Biotechniques.  10(4):506-13, 1991 Apr.                                    
>
>---
>Robert M. Horton
>http://134.84.47.3                        Have a :) day
>

While I haven't tried it PerkinElmer's publication "Amplifications : May 1989, Issue 2" has a method authored by Russel Higuchi.

Hope this helps



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