plant tissue grinding equipment
Michelle.Gleeson at SMTPGWY.AGRIC.NSW.GOV.AU
Michelle.Gleeson at SMTPGWY.AGRIC.NSW.GOV.AU
Tue Jan 9 18:07:25 EST 1996
Hi bionetters,
I'd like to hear people's recommendations on equipment for grinding
plant samples. I extract viroid RNA from leaves mainly, sometimes
bark. I have tried two blade type machines (Ultra Turrax and
Polytron), but these do not give small enough leaf particles and are
hard to clean between samples. It would be best if the equipment
could be cleaned with sodium hypochlorite. A Waring blender did not
work either. Mortar and pestle gives the required results eventually,
but I want to process large numbers of samples - painful! The tissue
needs to be a thin paste when ground with buffer, with none or few
recognisable leaf particles left. I've heard good stories about a
"Klecko" machine which uses ball bearings, but we'd need to import one
into Australia to even try it, I think (suppliers?, any in Oz?). Any
hints, recommendations etc gratefully accepted.
Thanks in advance
Michelle Gleeson
NSW Agriculture Biological and Chemical Research Institute
Sydney, Australia
gleesom at agric.nsw.gov.au
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