RNAse for phage preps
john.brennand at gbapr.pharmaceuticals.zeneca.tmailuk.sprint.com
Wed Jan 10 06:15:11 EST 1996
The crude stuff (Sigma) should be fine if treated appropriately - if
your DNA's are being degraded then either one of your other reagents or
your tubes, tips, etc are contaminated or you haven't got rid of the
You should always wear gloves when prepping DNA's - protecte your work
from you not you from the work !
Boil the RNAse solution for 15 min then spin it hard >10,000g to pellet
the precipitated DNAse - then aspirate about 2/3 of the supernatant to a
sterile tube and store at -20(in aliquots) don't be greedy (it is cheap
after all) or you may transfer some DNAse
I made some up like this in 1989 and it is still good today.
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