fresh transformations for protein expression??

Cornelius Krasel krasel at
Wed Jan 10 04:53:57 EST 1996

I made a mistake in my previous post :-(

Cornelius Krasel (krasel at wrote:
> If you cotransform BL21(DE3) with pLysS or pLysL the system becomes less
> leaky. BL21(DE3)pLysS are available from several companies; I don't know
> about BL21(DE3)pLysL (which is a stronger repressor than pLysS).


/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at  SP3 */
/* "Science is the game we play with God to find out what His rules are."  */

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