T/A cloning

Robert Horton horton at biosci.cbs.umn.edu
Wed Jan 10 13:38:32 EST 1996


Simon Dawson (Simon.Dawson at nott.ac.uk) wrote:

{...]
:   If anyone wants the method we use for making T-vector, it's Nucl. Acids. 
: Res., (1981) Marchuk et al. I can't remember the page number though....it's 
: only a one-page article.

1981? Wow, they were really ahead of their time.     :)

Marchuk, D., Drumm, M., Saulino, A., and Collins, F.S. Construction of
T-vectors, a rapid and general system for direct cloning of unmodified
PCR products. Nucleic Acids Res. 19:1154, 1991.


---
Robert M. Horton
http://134.84.47.3                        Have a :) day




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