Cacodylate & CoCl2

john brennand john.brennand at gbapr.pharmaceuticals.zeneca.tmailuk.sprint.com
Thu Jan 11 09:10:55 EST 1996


Chris

Back in the bad old days (1981!!) of tailing cDNA & plasmid for library 
production I saw a similar thing.

It was easily circumvented by passing the tailed cDNA or vector through 
a Sephadex G50 spin column prior to the next step - suggesting that one 
or more of the low molecular weight components was responsible for the 
pellet

I suggest that you desalt your reaction using any one of 20 or so 
commercial colums, kits, etc, before attempting to precipitate it





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