SSCP and glycerol

[ynakao at kean.ucs.mun.ca]

Hello, Netters,

    I have another question about PCR-SSCP. Making the gel, 5-10% of 
Glycerol is usually (not always) added in the sequencing gel contents.
What is the reason? Is it necessary or better than normal sequencing gel?

Thanks in advance

Yoshi