Stubborn DNA during resuspension

Richard Daines rdaines at
Thu Jan 11 18:47:39 EST 1996

I have run into this problem myself. I get excellent DNA yeilds (seen when 
I ppt, nice long spoolable DNA) but if I centrifuge, the pellet us almost 
totally insoluble.  I've always blamed co-ppt of carbohydrate as the 
problem.  I seem to be able to get enough back into solution to be useful 
and keep the rest as a pellet.  Sorry but I don't have a cure for this 

More information about the Methods mailing list