Stubborn DNA during resuspension

Richard Daines rdaines at q.continuum.net
Thu Jan 11 18:47:39 EST 1996


I have run into this problem myself. I get excellent DNA yeilds (seen when 
I ppt, nice long spoolable DNA) but if I centrifuge, the pellet us almost 
totally insoluble.  I've always blamed co-ppt of carbohydrate as the 
problem.  I seem to be able to get enough back into solution to be useful 
and keep the rest as a pellet.  Sorry but I don't have a cure for this 
problem.




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