Vent gives blunt end products? (was: Re: ligation of blunt PCR products)

Jan van der Ploeg ploeg at micro.biol.ethz.ch
Fri Jan 12 11:09:56 EST 1996


In article <svuilleu.3.000A16A8 at micro.biol.ethz.ch> svuilleu at micro.biol.ethz.ch (Stephane Vuilleumier) writes:
>From: svuilleu at micro.biol.ethz.ch (Stephane Vuilleumier)
>Subject: Vent gives blunt end products? (was: Re: ligation of blunt PCR
>products)
>Date: Fri, 12 Jan 1996 10:05:14
>Keywords: vent, blunt PCR

>In article <delabess-1101961947420001 at appp5.ext.infobiogen.fr>
>delabess at infobiogen.fr (Eric DELABESSE) writes:
>>From: delabess at infobiogen.fr (Eric DELABESSE)
>>Subject: Re: ligation of blunt PCR products
>>Date: Thu, 11 Jan 1996 19:47:42 +0000

>>Aplify your PCR with Vent polymerase (New Englands Biolabs) which gives
>>blunt PCR products which is different from Taq polymerase and clone in pCR
>>Script (Stratagene) which a blunt end cloning vector.

>I keep hearing/reading this, but never seem to be able to find a real 
>reference where this has been demonstrated experimentally  (maybe I haven't 
>look hard or carefully enough). Anybody  got such a reference?

>Thanks, Stephane

Hi Stephane,

Maybe this is what you are looking for:
Garrity and Wold. PNAS 89:1021-1025 (1992).

I will put a copy on your desk...

Jan van der Ploeg





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