pGEX system

meller yael meller at
Sun Jan 14 13:23:00 EST 1996

 I have the plasmid pGEX-2T containing gene insert. These gene doesn't
have his own sequence for transcription termination, but can be expressed
in the plasmid in frame with Sj gene (on Sj's 3'- terminus)! I would like to
reclone my gene together with Sj- transcription terminator from pGEX to
another vector. 
 So, any information about transcription termination for genes cloned in
pGEX or for Sj gene would be very appreciated! 
 Thanks in advance. Il. 
f139994 at

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