DEAE-transfection of EL-4 cells

Paul Boyer boyer at
Mon Jan 15 13:17:32 EST 1996

Hung-yiu Ho (hyho at GATE.SINICA.EDU.TW) wrote:
: Dear netter,
:   What is the optimal condition (i.e. DEAE-dextran concentration and 
: duration of DMSO shock) for EL-4 cells? I have tried very hard to test 
: it. Please help me!

Try Tom Novak, now at Wyeth-Ayerst.  As a graduate student, he very 
successfully worked out the conditions and published the work in Mol. 
Cell. Biol.  Ask him for the reference.  His address is 
novakt at 

Paul D. Boyer, Ph.D.                      University of Wisconsin--Parkside
Assistant Professor                       900 Wood Road, Box 2000
Department of Biological Sciences         Kenosha, WI  53141-2000

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