DEAE-transfection of EL-4 cells

[Paul Boyer]

Hung-yiu Ho (hyho at GATE.SINICA.EDU.TW) wrote:
: Dear netter,
:   What is the optimal condition (i.e. DEAE-dextran concentration and 
: duration of DMSO shock) for EL-4 cells? I have tried very hard to test 
: it. Please help me!

Try Tom Novak, now at Wyeth-Ayerst.  As a graduate student, he very 
successfully worked out the conditions and published the work in Mol. 
Cell. Biol.  Ask him for the reference.  His address is 
novakt at prince.mm.wyeth.com 

===========================================================================
Paul D. Boyer, Ph.D.                      University of Wisconsin--Parkside
Assistant Professor                       900 Wood Road, Box 2000
Department of Biological Sciences         Kenosha, WI  53141-2000
============================================================================