BrdU in immunoflourescence
aschoen at aecom.yu.edu
Mon Jan 15 19:31:05 EST 1996
Hi. I want to do double staining IF for my protein and for cell
proliferation with BrdU. Optimally, I would like to detect BrdU with a
non-rabbit primary antibody and a Texas red or rhodamine conjugated
secondary antibody (because I have had success detecting my protein with
FITC). My questions are directed to anybody who has had success with
1) Which companies have good reagents to perform this experiment?
2) Is there a good protocol for this procedure? It seems a little more
complex than IF as you have to make the incorporated BrdU's accessible
to antibody (i.e. nucleases, etc.).
Thanks for your help.
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