soluble protein expression in E. coli
r.seymour at prospect.anprod.csiro.au
Tue Jan 16 21:44:24 EST 1996
In article <4dgu30$j6a at lyra.csx.cam.ac.uk>,
sb at mole.bio.cam.ac.uk (Saverio Brogna (Genetics)) wrote:
>I have expressed a Drosophila protein in E. coli by using several
>strategies (unfused, fused to glutation transferase, and fuse to 6-His
>tag) but all of it is unsoluble.
>DO YOU KNOW OF ANY WAY TO EXPRESS PROTEINS IN E. COLI THAT ARE SOLUBLE?
Welcome to the club of people expressing eukaryotic proteins in E. coli that
turn out to be insoluble. There is some published literature around (though
I can't remember any off the top of my head) that talk about the use of
chaperones expressed in conjunction with insoluble eukaryotic proteins to
improve their folding in E.coli and thus solubility. These chaperones are
called (I think)gro E and gro L and expression sytems that use them are
available commercially (I think).
CSIRO Division of animal production
(The usual disclaimer)
More information about the Methods