T/A cloning again. To Kinase or Not

Dr. Duncan Clark duncan at genesys.demon.co.uk
Tue Jan 16 10:43:53 EST 1996

In article <4dg4u5$3cc at bisance.citi2.fr>, Francois-Xavier Maquart
<maquart at bisance.citi2.fr> writes

>At the begining I have a naive question: Should I kinase PCR products
>(or oligos) for T/A cloning? Either Promega pGEM-T vector system kit
>or Invitrogen TA cloning system kit do not discuss this problem.

No need to kinase as there is a 5' phosphate already on the TA vector.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

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