T/A cloning again. To Kinase or Not

Michael Dalrymple dalrymple at pplros.demon.co.uk
Tue Jan 16 10:39:33 EST 1996

In article <4dg4u5$3cc at bisance.citi2.fr> Francois-Xavier Maquart,
maquart at bisance.citi2.fr writes:
> Should I kinase PCR products
>(or oligos) for T/A cloning? Either Promega pGEM-T vector system kit
>or Invitrogen TA cloning system kit do not discuss this problem.

Put simply, yes.


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