PCR from single colonies?

CK Wen cwen at aux.btny.purdue.edu
Tue Jan 16 09:26:39 EST 1996


Nathan Lange <nelange at ucdavis.edu> wrote:
>Does anyone have a good protocol or advice for doing PCR directly from 
>single bacterial colonies?
>
>thanks in advance,
>
>Nathan
>
>
Dear Nathan:
  To do this, just use a tooth pick to pick E.coli from a colony and
transfer it to a tube which has reagents mixed for PCR.

Chi-Kuang





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