Quantitating Northerns on MD PhosphorImager

Patrick HJ Falckh p.falckh at rmit.edu.au
Wed Jan 17 23:54:47 EST 1996


I agree with Benjamin.  It is better to turn off the background 
subtraction routine and select another appropriate area to determine the 
background.

As to actin, and I'm assuming you mean beta-actin, this is modulated in 
some systems; in cardiac tiisue the expression is altered when looking 
at the effects of agonists/antagonist that will result in changes in 
hypertrophy and hyperplasia (size and cell numbers).  If this occurs in 
one tissue the same could hold true for other tissue.  I suppose the 
best method for quantitation is the use of MORE than one standard for 
comparison.  Every protein will be effected in a fluctuating system and 
the only hope is to use that which changes the least.


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