T/A cloning again. To Kinase or Not

[HIRANYA ROYCHOWDHURY]

On 16 Jan 1996, Francois-Xavier Maquart wrote:

> Dear Netters.
> 
> I have just discover this newsgroup. Fascinating.
> At the begining I have a naive question: Should I kinase PCR products
> (or oligos)
Yes, phosphorylating will definitely better the chance.

 for T/A cloning? Either Promega pGEM-T vector system kit
> or Invitrogen TA cloning system kit do not discuss this problem.
> 
> Thank you in advance.
> 
> Yanusz
> 
> (e.mail: maquart at citi2.fr )
> 
> 
> 


			>>>>>>>>>>>>>>>>>>>>>>>>>>>
			  Hiranya S. Roychowdhury
   			  Plant Genetic Engineering Lab.
			  Box 3GL, NM State Univ.
			  Las Cruces, NM 88003
			  Phone: (505) 646-5785
			  hroychow at nmsu.edu
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