T/A cloning

W.Coco ifai at po.uni-stuttgart.de
Wed Jan 17 10:18:40 EST 1996


In article <4cr5d7$ue0 at news.doit.wisc.edu>, Helkor at facstaff.wisc.edu (Helen Korsmo) says:
>
>We ligated an insert into a T-vector (after treating the insert for 30 minutes with Taq; in order to 
>put A's on the ends) and transformed DH5-alpha cells and plated out on LB plate with IPTG and 
>X-gal.  We got several white colonies ( presumably these have insert in the vector), but mostly 
>blue (vector alone).  Is it possible for T-vectors to ligate?
>


Why not dephosphorylate the vector? Wouldn't this cut down on auto-
ligations?

Wayne



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