Is linear DNA mandatory for PCR amplification ??
tomlinson at pplros.demon.co.uk
Wed Jan 17 09:45:39 EST 1996
I remember a discussion here more than a year ago on why sequencing was
with supercoiled plasmid (don't remember why!) but PCR wasn't. I had
using a supercoiled template which would not amplify, and so I linearised
and the PCR promptly worked perfectly. Mere anecdotage, but it worked
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