Is linear DNA mandatory for PCR amplification ??

Anthony Tomlinson tomlinson at pplros.demon.co.uk
Wed Jan 17 09:45:39 EST 1996


I remember a discussion here more than a year ago on why sequencing was
best
with supercoiled plasmid (don't remember why!) but PCR wasn't.  I had
previously been
using a supercoiled template which would not amplify, and so I linearised
it
and the PCR promptly worked perfectly.  Mere anecdotage, but it worked
for me.

Anthony.



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