In article <OQYWSDA5e8+wEwIB at genesys.demon.co.uk> Dr. Duncan Clark, duncan at genesys.demon.co.uk writes: >No need to kinase as there is a 5' phosphate already on the TA vector. > Oops. Apologies to Francois, and to Mike Dalrymple, whose name I used mistakenly in my last, incorrect post. Anthony.