Is linear DNA mandatory for PCR amplification ??

Volker Blaschke vblasch at gwdg.de
Thu Jan 18 02:43:21 EST 1996


No, PCR from cDNA in plasmids works perfectly well, at least for me...
I use an initial 3 min. at 95 C and 1 min 95 C in every cycle.

Good luck,

  Volker





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