T/A cloning again. To Kinase or Not

[David Micklem]

In article <Pine.SOL.3.91.960117173641.17588A-100000 at howard>, Rafael
Maldonado <rafael at howard.genetics.utah.edu> wrote:


>I don't get the point. Kinase what? PCR product, as it is, is already 
>kinased (I mean that it has a phosphate at the 5' end).
>
Is it?  If you don't kinase your oligos (I don't) when exactly does your
PCR product acquire its 5' phosphate? 

David

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