Plasmid copy number determination

Edgar Valencia edgar at
Thu Jan 18 18:11:34 EST 1996


I dont know which protocol are you using for plasmid copy number 
determination, but why dont you take a look to the Shields et al
protocol J. Microbiol. Methods (1986) 6:33-46 as well as to the
modification made by Durland and Helinski J. Bact. (1990) 172:
3849-3858. Perhaps works for you.

I hope this helps 

                          Edgar Valencia Morales
                    Departamento de Genetica Molecular
                               CIFN - UNAM
                        e-mail edgar at

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