PCR from whole blood (PE/ABI GeneScan)
A.P.R.M. OSTEROP at TCH
OSTEROP at tch.fgg.eur.nl
Fri Jan 19 03:33:08 EST 1996
In article <4dfoqn$96a at erika.cica.es> .bb1dopeg at lucano.uco.es (bb1dopeg) writes:
>From: .bb1dopeg at lucano.uco.es (bb1dopeg)
>Subject: PCR from whole blood (PE/ABI GeneScan)
>Date: 16 Jan 1996 08:49:27 GMT
>Anyone doing PCR from whole blood?
>Any hints most appreciated.
>bb1dopeg at uco.es
I have been using GeneReleaser, a matrix that binds PCR inhibiting stuff
present in blood, from BioVentures. You add it to 1 microliter blood, do a
specified thermocycler procedure, and then add your amplification reagents
and start PCR. Very easy and it works fine in my hands. However, if you
are concerned about cross-contaminations you must realise UDG will become
inactivated by this matrix too.
Erasmus Univ. Rotterdam
osterop at tch.fgg.eur.nl
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