SSCP

Dominic Voon dvoon at cyllene.uwa.edu.au
Fri Jan 19 00:42:01 EST 1996


It could also be due to the fact that the ssDNAs assume more than one 
stable conformation under the conditions you ran the gel. When I was doing 
'cold' SSCP, I was able to remove these ghost bands by lowering the 
electrophoresis temperature to 4 C. In my (limited) experience, these bands 
can also result from partial denaturation. In some cases, I detect a 
smear of ghost bands using NaOH as the denaturant but not so when using 
methylmercuric hydroxide. It's probably a combination of these 
factors, but the former is likely to be more significant.

Anyway, just my $0.02's worth. Hope you'll find it useful.

regards
dom 



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