Plant genomic DNA extraction protocol?

Paul E. Ulanch PULANCH at bioch.tamu.edu
Fri Jan 19 14:42:06 EST 1996


In article <4dn7rn$3nl at news.iastate.edu>,
   ckafer at iastate.edu (Chris Kafer) wrote:
>I need a quick "miniprep" protocol for genomic DNA extraction from
>plant tissue (~ 1.0 gram samples).  The DNA will be used for
>restriction digests,  blotting etc.
>
>Any and all help appreciated.  Thanks in advance!
>
I have tried this for use in RAPD analysis with sorghum DNA.  It is 
quick, fairly cheap, uses no organics, and gives high yields 
compared to the starting amounts.  It uses PEX.  Look up Nucl Acids 
Research 22(10):1917.  

Others use it for arabidopsis extractions with great luck!  Hope it 
works,
 

Paul "Goose" Ulanch

Dept. Biochem/Biophys
Texas A&M University
College Station, TX   77843-2128
(409) 847-9379
PULANCH at bioch.tamu.edu



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