Effect of LiCl on RT and enzymes

spyong at pc.jaring.my spyong at pc.jaring.my
Sat Jan 20 01:49:03 EST 1996


I have been trying to construct a cDNA library but failed. I was told that
LiCl used to percipitate my RNA is affecting the enzyme RT.Is this true? I'm
thinking of using this same sample to do RACE-PCR(Frist attempt!...). Will I
have difficulties too?

Please help me.
Thanks.
Stephanie




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