Tiny amount mRNA
ynishiwatari at UCDAVIS.EDU
Fri Jan 19 19:42:56 EST 1996
I am planning to make cDNA library from tiny amount of mRNA, less then 10 ng. Does
anybody have any experience for ampification of small amount of mRNA? Or does anybody
know good protocol for that kind of experiment? In case It's necessary, I can try to increase
the amount of mRNA little bit more up to 30 ng. But that's the maximam I can prepare.
Any idea would be very wellcome.
Thanks in advance, and good luck!
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