Tiny amount mRNA

Yutaka Ishiwatari ynishiwatari at UCDAVIS.EDU
Fri Jan 19 19:42:56 EST 1996

Dear Net-users,
I am planning to make cDNA library from tiny amount of mRNA, less then 10 ng. Does 
anybody have any experience for ampification of small amount of mRNA? Or does anybody 
know good protocol for that kind of experiment? In case It's necessary, I can try to increase 
the amount of mRNA little bit more up to 30 ng. But that's the maximam I can prepare. 

Any idea would be very wellcome.
Thanks in advance, and good luck!


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