DNA fixation on Agarose gel
Mike Gruidl
mgruidl at com1.med.usf.edu
Sat Jan 20 09:12:00 EST 1996
In article <pcomm.190.0010FBF9 at cismibm.univ-lyon1.fr>,
pcomm at cismibm.univ-lyon1.fr says...
>
>Hello
>
>I am looking for a method to fix DNA in agarose gel after
electrophoresis.
>The classical methods (formaldehyde, glutaraldehyde....) don t works in
>agarose gel.
>
>Please, help me !
>
>Merci
>
>Pierre
>Pcomm at cismibm.univ-lyon1.fr
Try 7% TCA for 30 minutes. I used to use this and then flatten the gel
under paper towels and a weight. For more detailed references I think
there might be something in Maniatis cloning manual.
Mike Gruidl
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