T/A cloning again. To Kinase or Not

[Jonathan Hecht]

Francois-Xavier Maquart wrote:
> Should I kinase PCR products
> (or oligos) for T/A cloning? Either Promega pGEM-T vector system kit
> or Invitrogen TA cloning system kit do not discuss this problem.

You don't have to kinase the PCR product since the 5' phosphates are supplied 
by the vector, thus it works like any other ligation where you 
dephosphorylate one of the DNA fragments.

Jonathan Hecht