T/A cloning again. To Kinase or Not
jhecht at ucsd.edu
Mon Jan 22 00:38:37 EST 1996
Francois-Xavier Maquart wrote:
> Should I kinase PCR products
> (or oligos) for T/A cloning? Either Promega pGEM-T vector system kit
> or Invitrogen TA cloning system kit do not discuss this problem.
You don't have to kinase the PCR product since the 5' phosphates are supplied
by the vector, thus it works like any other ligation where you
dephosphorylate one of the DNA fragments.
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