Concealed (Genequant II)
yacman at homer07
Sun Jan 21 19:32:05 EST 1996
Steve Stelman (steves at burn.ucsd.edu) wrote:
: John Hachey wrote:
: > Subject: Genequant II-comments?
: > We are thinking of purchasing a GeneQuant II unit primarily for
: > DNA and RNA quantitation.
: > Can some owners of this machine share their reviews of this
: > instrument?
: > Thanks, John.
: In short, here are a few of the pro's and con's
: -more accurate quantitation than using a spec
I believe it is, in fact, a limited wavelength spec.
: -easier than our fluorometer, which is difficult to zero
: -light source does not need to warm-up before use
: -calculates concentrations based on A260 and A280 in single read
But does not give a curve to distinguish other possible contaminants in
the solution as a spec wavelength scan can. Furthermore, a Genequant will
not distinguish RNA from DNA if RNA might contaminate your preps. A
fluorometer will. Unfortunately, a Fluorometer can't be used for RNA,
oligos, or DNA samples that have been exposed to ethidium. The
single greatest advantage of a fluorometer is that it is 100-1000 times
more sensitive than a spec. Useful for conserving precious sample.
: -has many calculation functions built in
: -cuvette not included (expensive to purchase from them)
I don't know. Probably true.
: -cuvette must be inserted and removed on cue from machine
: within a few seconds, or reading is aborted. This
: gets to be REALLY annoying, as you have to hover
: over the machine with the cuvette waiting for the
Yeah. That is irritating.
: Nevertheless, I use this machine regularly as opposed to
: the alternatives.
: Steve Stelman
: steves at burn.ucsd.edu
I use both, as the occasion warrants.
L. Scot Bastian Ph.D. 206-762-1010 ext. 1918 (voice)
University of Washington at the 206-764-2689 (FAX)
Veterans' Affairs Medical Center yacman at u.washington.edu (Email)
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Seattle WA 98108
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