Glutathione-HRP conjugate

Robert Slany rslany at leland.stanford.edu
Tue Jan 23 01:09:06 EST 1996


Hi George,
I wouldn't do this. Active gluthathione-S-transferase (that is binding to 
glutathion) is a dimer! So if your GST fusion protein "probe" is bound to 
your target protein, and therefore immobilized, it might inhibit dimerization 
and equally screw a binding to glutathion. 
If you want to try you can couple the molecules by carbodiimide (sorry no 
protocol at hand but the Methods of Enzymology series has tons of it). 

Just my 0.02$

Robert



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