rslany at leland.stanford.edu
Tue Jan 23 01:09:06 EST 1996
I wouldn't do this. Active gluthathione-S-transferase (that is binding to
glutathion) is a dimer! So if your GST fusion protein "probe" is bound to
your target protein, and therefore immobilized, it might inhibit dimerization
and equally screw a binding to glutathion.
If you want to try you can couple the molecules by carbodiimide (sorry no
protocol at hand but the Methods of Enzymology series has tons of it).
Just my 0.02$
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