Please help me to reamlify RAPD band

Bixing Huang huangbx at DEAKIN.EDU.AU
Mon Jan 22 17:59:06 EST 1996


Dear netters,

I am trying to reamplify a RAPD band for cloning. Every time I cut the band
from gel and wash it very carefully then diffuse it out. Take the
supernatant as template and to run the PCR using the same random primer and
cycles down to 30. When it run on the gel, the reamplified shows 2-8 bands.
Do you have any good idea to get a single band?

Thank you for the help in advance.

Ben Huang
huangbx at deakin.edu.au
Deakin University of Australia 
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