magnetic bead losses during manipulation

W.Coco ifai at po.uni-stuttgart.de
Tue Jan 23 08:52:43 EST 1996


Paul, you mentioned how you siliconize with Sigmacote, but didn't indicate
a washing step (as does Maniatis for dichlorodimethylsilane). Is this
not necessary only with your method or would you also not rinse after
Maniatis' method (p. E.1 in the 2nd ed.)? If not, the Maniatis method,
performed in a vacuum desicator, allows
one to treat lots at once (of course they warn you could destroy your
vacuum pump if not careful).

Thanks,

Wayne



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