DNA stuck in agarose gel wells

John Ladasky ladasky at leland.Stanford.EDU
Tue Jan 23 14:52:37 EST 1996


In article <boehnin1-2301961430170001 at joseph2.tjh.tju.edu>,
Darren Boehning (or is it Shaila Bokkala? see below)
<boehnin1 at jeflin.tju.edu> wrote:
>I have been experiencing problems with DNA being stuck in the wells.
>Although we do find that some of the sample comes out and is seen as a
>faint band but most of the remains in the well.The well is not
>contaminated with pieces of agarose. The wells are well formed too. I
>would appreciate any suggestions.
>
>Shaila Bokkala
>Thomas Jefferson University
>E-mail at Bokkala1 at jeflin.tju.edu

	Is this genomic DNA?  How is the DNA prepared? 

-- 
Unique ID : Ladasky, John Joseph Jr.
Title     : BA Biochemistry, U.C. Berkeley, 1989  (Ph.D. perhaps 1998???)
Location  : Stanford University, Dept. of Structural Biology, Fairchild D-105
Keywords  : immunology, music, running, Green



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