SDS-PAGE:slower migration after reducing?

John Edward Smith jes2u at faraday.clas.Virginia.EDU
Tue Jan 23 21:32:24 EST 1996



Any ideas why the same sample treated with the same loading buffer (2X conc:
 0.125 M Tris pH 6.8, 4% SDS, 20% glycerol) either (1) without beta-
mercaptoethanol and without boiling or (2) with BME (5% final conc) and
 boiling 10 minutes results in a slower migration under reducing/denatureing
conditions?




Many thanks
-John Smith




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