Is linear DNA mandatory for PCR amplification ??

Ed Castro ecastro at
Tue Jan 23 17:04:29 EST 1996

There should not be any problem PCRing from plasmids....I have done 
it with no problem in order to sequence, amplify, make probes, etc.

The only difference bet amplifying from genomic v plasmid has to do 
with the starting template concentration, obviously you need a  lot 
less when amplifying from plasmid.

More information about the Methods mailing list