Shortened products with pET vector

LSolo77049 lsolo77049 at
Wed Jan 24 05:04:10 EST 1996

Check the codon usage of the insert and compare it to the E coli high
expression list. Sometimes a group of codons used a low efficiency in E
coli (some Arg and Pro codons and others), the protein will prematurely
fall of the ribosomes.

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