RNA extraction from < 50 mg

[Chi-Kuang Wen]

Ed Castro <ecastro at physci.ucla.edu> wrote:
>We will be doing RNA extractions from fetal rat hearts, we will 
>probably be dealing in the range of 10-50mg.  Does anybody have a 
>protocol that works well for this amounts of tissue?  Or can someone 
>recommend a kit that is reasonably priced? (do not want to pay 
>Invitrogen prices)
>
>Thanks to those of you who have already answered....


  A way is to scale down the amount of buffer used for general 
RNA isolation.  I have succeeded in isolating RNA from plant
cells(germinating fern gametophytes) in a range of 50 to 100mg 
of gametophytes.


Chi-Kuang

-------------- next part --------------
From: Ed Castro <ecastro at physci.ucla.edu>
Reply-To: ecastro at physci.ucla.edu
Newsgroups: bionet.molbio.methds-reagnts
Subject: RNA extraction from < 50 mg
Date: Wed, 24 Jan 1996 16:53:38 -0700
Organization: UCLA
Mime-Version: 1.0
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Message-ID: <3106C682.795C at physci.ucla.edu>

We will be doing RNA extractions from fetal rat hearts, we will 
probably be dealing in the range of 10-50mg.  Does anybody have a 
protocol that works well for this amounts of tissue?  Or can someone 
recommend a kit that is reasonably priced? (do not want to pay 
Invitrogen prices)

Thanks to those of you who have already answered....