Sense-Antisense, genuine confusion
Harry J. Witchel
Harry.Witchel at bristol.ac.uk
Wed Jan 24 08:06:36 EST 1996
I feel like I am writing one of those ridiculous letters to a porno
magazine beginning with the words: I didn't ever think it could happen to me,
but one day...
I am trying to make an RNA transcript for injection into Xenopus
oocytes. I have the gene in bluescribe (Ks+) in a version in which the ATG
codon (going 5' to 3') is near the Bam site and what is labelled as a T7
initiator. At the other end is a Hind3 site and a T3 initiator.
I just want to make sure this is correct: the T7 will start
transcribing on the DNA strand without the ATG, such that the final RNA
product has the ATG, and this product will be what I want to inject.
The thread that keeps going and going
Harry.Witchel at Bristol.Ac.Uk
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