Lysing Yeast

Mark Farinha farinha at cas1.unt.edu
Wed Jan 24 10:36:50 EST 1996


In article <4e50d1$l2j at newsbf02.news.aol.com>, lsolo77049 at aol.com (LSolo77049) says:
>
>I'm looking for an efficient, reproducible method to lyse yeast
>(Saccharomyces cerevisae) for purification of native proteins. I'd like to
>try enzymes such as Zymolyase but can not find a vendor. I've tried glass
>beads in vortexing (small scale) and bead beaters as well as with
>sonication. All work, but they are not as reproducible as I'd like.
>Please send suggestions (and/or vendors for the enzymes).
>Thanks in advance,
>Larry Solomon


Sigma sells Lyticase which works fairly well on S.C. and C. albicans



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