RNA extraction from < 50 mg
paquetty at ERE.UMontreal.CA
Fri Jan 26 12:20:17 EST 1996
Chi-Kuang Wen <cwen at aux.btny.purdue.edu> writes:
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>Ed Castro <ecastro at physci.ucla.edu> wrote:
>>We will be doing RNA extractions from fetal rat hearts, we will
>>probably be dealing in the range of 10-50mg. Does anybody have a
>>protocol that works well for this amounts of tissue? Or can someone
>>recommend a kit that is reasonably priced? (do not want to pay
>>Thanks to those of you who have already answered....
> A way is to scale down the amount of buffer used for general
>RNA isolation. I have succeeded in isolating RNA from plant
>cells(germinating fern gametophytes) in a range of 50 to 100mg
I once had to isolate RNA from the thymus of 15 day old mouse embryos.
I dont know how much they weigh, but they are quite small. I just
used the standard Chomzinsky and Sacchi method, but i scaled down all
the volumes so that everything could fit in a 500ul tube. It worked
fine, and i got enough RNA to get nice results in an RNase protection
Universite de Montreal.
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