Shortened products with pET vector

[MV. Hayes]

This could be a result of secondary site translation initiation which can 
happen if a sequence in the RNA coding sequence resembles the ribosome 
binding site (AAGGAGG) and occurs 5-13nu 
upstream of the Met codon.
 References: Gene 72 p179-186 and Biotechnology 
3, p715-20.
Hope this helps

 Clinton Chapple (chapple at biochem.purdue.edu) wrote:
:      I have been having trouble with shortened products after expression 
: in pET32 and heard that others have had similar problems.  Could anyone 
: offer advice on this problem?


--

Dr Michelle Hayes
Department of Biochemistry
University of Bristol