Shortened products with pET vector

MV. Hayes bimvh at zeus.bris.ac.uk
Fri Jan 26 10:00:22 EST 1996


This could be a result of secondary site translation initiation which can 
happen if a sequence in the RNA coding sequence resembles the ribosome 
binding site (AAGGAGG) and occurs 5-13nu 
upstream of the Met codon.
 References: Gene 72 p179-186 and Biotechnology 
3, p715-20.
Hope this helps

 Clinton Chapple (chapple at biochem.purdue.edu) wrote:
:      I have been having trouble with shortened products after expression 
: in pET32 and heard that others have had similar problems.  Could anyone 
: offer advice on this problem?


--

Dr Michelle Hayes
Department of Biochemistry
University of Bristol




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