Prolonged radio-labeling with 35S-Methionin - any effect?

G.P. goran at
Fri Jan 26 07:44:22 EST 1996

Does anybody have any experience with radio-labeling of proteins with 
35S-methionin for several days (instead maximum overnight)?

Is there any increase in sensitivity when one compares 4 hr - overnight
- 3 day labeling?

What kind of media would you recommend for this?

I am using 100 uCi/ml 35SMeth in 

90% (Meth-free MEM) + 10% (MEM with Meth and 10% FCS) for overnight
Any suggestions?



goran at

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