Is Insect 28 and 18S banding different ?
mbxnjk at vax.nott.ac.uk
Wed Jan 24 07:00:51 EST 1996
Edwin Stephenson wrote:
> Drosphila 28s rRNA, and presumably many 28s rRNAs, are intact when they
> are first assembled into ribosomes, but cleaved by an unknown mechanism
> into two fragments sometime after incorporation into the ribosome. This
> cleavage site is almost exactly in the middle of the 28s molecule, and
> the two halves each run at the same mobility as 18s rRNA. So if you run a
> completely denaturing gel, and can resolve them, you should see three
> very closely spaced bands - 18s and the two pieces of the 28s. If your
> gels are not completely denaturing, you may see some full-size 28s rRNA.
> This has proably been nicked but the two halves are held together by
> intramolecular base pairing. So you aren't doing anything wrong.
Yes, same in the Tobacco Hawk Moth Manduca Sexta !
Appears like one 18S band !
|\ | | / \ / | NIGEL KENWARD,
| \ | | | ___ |__ | Dept. Biochemistry,
| \ | | | | | | Nottingham University Medical School.
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